Biochemistry Answers

1. Pseudomonas putia is thought to degrade hydrocarbons due to the fact that it harbours a TOL plasmid. To verify this assertion, a student was tasked to isolate and grow this soil-dwelling bacteria and to also isolate the plasmid from the microbe. The TOL plasmid is also intended to be used as a cloning vector.
A. Elucidate two physical properties that will enable you to isolate TOL plasmid from the chromosomal DNA of the bacteria.
B. Describe an appropriate method you could use to isolate TOL plasmid from the bacteria.
C. During the isolation of TOL plasmid from Pseudomonas putida, it was observed that the concentration of the plasmid DNA per 50 mL of sample was extremely low. Describe a technique you could employ to increase the concentration of the TOL plasmid.
D. Demonstrate a procedure that you could adopt to restrict or digest the TOL plasmid.
E. How would you ascertain if your restriction experiment was successful?

Demonstrate how you could isolate and culture Pseudomonas putida from soil sample contaminated with hydrocarbons

1. The application of organic matter to the soil improves soil quality. Discuss

2. i. Describe the structure of any two tectosilicates ,minerals found in soils.

ii. State the impact of these minerals on soils.

3. Isomorphous substitution is mechanism for the creation of charges on soil minerals. Explain

What happens to Glycerol released from triacylglycerols after lipase action? How does β-Oxidation of Fatty Acids to provide energy?

A fat has an iodine value of 250, what can you conclude about the nature of the fat? Can this fat be provided to a patient having cardiovascular disease?

what is operating temperature of clean bench in laboratory?
one colony represents how many cells?
the colonies of bacteria cell can be observed by?

After a person eat a meal rich carbohydrates,the digested sugars diffuse into the bloodstream and increase the level of sugar in blood.The pancreas release insulin in response to the increased blood sugar level.Insulin signals body cells and the liver to take up sugar from the blood, causing blood sugar level to decrease.When the blood sugar levels return to normal, the pancreas stop releasing insulin.Is this a positive or negative feedback mechanism

Are the reactions pyruvate to ethanol and pyruvate to lactate both reversible. If not, why not?

1. Consider a liver cell carrying out the oxidation of glucose under aerobic conditions. Suppose that we added a very potent and specific inhibitor of the mitochondrial ATP synthase, completely inhibiting this enzyme. Indicate whether each of the following statements about the effect of this inhibitor is true or false; explain in a sentence or two why. [10 pts]

____ (A) ATP production in the cell will quickly drop to zero.





____ (B) The rate of glucose consumption by this cell will decrease sharply.





____ (C) The rate of oxygen consumption will increase.





____ (D) The citric acid cycle will speed up to compensate.

why is hydrogen breath test done