Why must the restriction enzyme used for the digestion of TOL plasmid be inactivated immediately after the restriction experiment? How could this be achieved?
Why must the restriction enzyme used for the digestion of TOL plasmid be inactivated immediately after the restriction experiment? How could this be achieved?
During the isolation of TOL plasmid from Pseudomonas putida, it was observed that the concentration of the plasmid DNA per 50 mL of sample was extremely low. Describe a technique you could employ to increase the concentration of the TOL plasmid.
d. During the isolation of TOL plasmid from Pseudomonas putida, it was observed that the concentration of the plasmid DNA per 50 mL of sample was extremely low. Describe a technique you could employ to increase the concentration of the TOL plasmid.