Identify the function of enzymes in the digestive system. Explain how the process of digestion would be affected if enzymes were not present, Identify which enzyme is in each of the stock containers, and justify your choices. Construct a written explanation to describe how the scientists can identify the digestive enzymes.
In a nutrient medium that lacks histidine, a thin layer of agar containing around 10^9 Salmonella typhimurium histidine auxotroph produces around 13 colonies over a two day incubation period at 37 degrees centigrade. How do these colonies arise in the absence of histidine? The experiment is repeated in the presence of 0.4 micrograms of 2-aminoanthracene. The number of colonies the number of colonies produced over 2 days exceeds 10,000. What does this indicate about 2- anthracene? What can you surmise about its carcinogenicity?
a. During the isolation of TOL plasmid from Pseudomonas putida, it was observed that the concentration of the plasmid DNA per 50 mL of sample was extremely low. Describe a technique you could employ to increase the concentration of the TOL plasmid.
What are the drawbacks of immunoturbidimetry assays if compared to spectrophotometeric assays like cholesterol or glucose test?