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The ability of hemoglobin (Hb) to carry oxygen throughout the body as oxyhemoglobin (HbO2) is dependent on the pH of the blood. What effect would acidosis have on the ability of a patient to transport oxygen?


Why is the PI of human insulin higher than PI of duck insulin


What would happen to an organism that lacked the enzyme that catalyzes the first step in glycolysis? 


Calpain 1 is a cysteine-protease existing as a heterodimer made up of a large and small subunit. The large subunit is 714 residues long (mass 81.9 kDa) with a pI of 5.49. The small subunit is 268 residues long (mass 28.3 kDa) with a pI of 5.05. Calpain 1 is inhibited by calpastatin, an intrinsically disordered protein of 708 residues (mass 76.5 kDa) with a pI of 4.97. Suggest a purification method that would allow you to separate calpain 1 from calpastatin. Describe conditions and explain why the chosen method would work and give reasons why it wouldn’t work.


Illustrate the titration curve of lysine showing the cation, zwitterion and anion on the curve

Write the structure and function of coenzyme form of the vitamin attached to the acyl carrier protein


When bacteria growing at 20°c are warmed at 30°c are more likely to synthesize membrane lipids with saturated or unsaturated fatty acid. Explain
Mutations within the DNA sequence of an organism can
Estimate the concentration of an enzyme in a living cell. Assume that fresh tissue is 80% water and all of it is intracellular, the total soluble protein in a cell represents 15% of the weight, all the soluble proteins are enzymes, the average weight of a protein is 150000 and about 1000 different enzymes are present.
1. Calculate the isoelectric point of glutamic acid. How does the isoelectric point of glutamic acid differ from glycine.
2. Illustrate the titration curve of lysine showing the cation, zwitterion and anion on the curve.
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