Biochemistry Answers

Given the overall equation for respiration (C₆H₁₂O₆ + 6O₂ 6CO₂ + 6H₂O), in which step(s) in the respiratory pathway is/are each of the reactants used up and each of the products made? 

Mitochondrial encephalomyopathy, lactic acidosis and stroke-like episodes (MELAS) is a mitochondrial inherited disorder due to deficiency of either NADH/COQ reductase (Complex I cytochrome oxidase (Complex IV). Due to this lack of a functional mitochondria, what would the net ATP produced from one molecule of glucose? 6+2=8.

A. 0

B.1

2

D. 4

E. 8

Why would the pH of your blood plasma increase if you breathe fast?

Give an account of the total ATP yield when I molecule of glucose convert carbon dioxide and water

- Briefly explain why we incubate the purified plasmid with the restriction enzymes, BamHI and XhoI, at exactly 37oC. 

Assume the linear fit of the standard curve using DNSA absorbance at 540nm versus glucose concentration in M is y=0.002x + 0.003. Calculate the concentration of an unknown solution of glucose with DNSA absorbance at 540nm of 0.17.

What is the importance of nucleic acid studies in elucidation and treatment of diseases

State three errors of nucleic acid metabolism, note in each case the defective enzyme

Explain in NOT MORE than 350 words how giving an alcoholic patient who has hypoglycaemia an IV infusion of glucose ALONE can be fatal.

Explain in NOT MORE than 350 words how giving an alcoholic patient who has hypoglycaemia an IV infusion of glucose ALONE can be fatal.