Under most in vitro assay conditions, the enzyme is used in catalytic amounts[10^(-12) to 10^(-8) M]. Estimate the concentration of an enzyme in a living cell. Assume that-
i) fresh tissue is 80% water and all of it is intracellular.
ii) the total soluble protein in a cell represents 15% of the wet weight.
iii) all the soluble proteins are enzymes.
iv) the average molecular weight of a protein is 150,000 and
v) about 1000 different enzymes are present.