Answer to Question #95811 in Biochemistry for MONAAMBIGHAI

Question

What do you think the accuracy level of DNS Assay for glucose measurement?

Answer 1

 Accuracy Level of DNS Assay for Glucose Measurement

           DNS assay is more accurate above 50mg/ml dosage of glucose . The accuracy is usually weak below bellow 50mg/ml. For reducing sugars, glucose concentrations of 0-1mg/ml and 0-25mg/ml are used. The reason behind this is that high concentrations shows the same color range whereas in lower concentrations, there is a notable change. However, DNS assay method is associated with several shortcomings hence it is not the most appropriate for glucose measurements.

                                   Reference

Souhad El Akoum;<sensitivity of DNS method for glucose determination>.https://www.researchgate.net/post/Is- anybody- familiar- with- the- sensitivity- of- DNS- method- for- glucose- determination.