Answer to Question #49039 in Biochemistry for Peter martin

Question #49039
which of the following altrations of protein structure would have least affect on the ability of a protein to function as an enzyme?
a) alkylation of single Cys residue of protine with iodoacetate.

b)site-directed mutagenesis in which an Ile in the interior of the protein is replaced by a V al

c) Amidation of all Glu to Gln

d) faliure to remove the N- terminal hydrophobic peptide of all protein during processing
1
Expert's answer
2014-11-19T11:04:46-0500
b. substitution I->V is less likely to affect enzymatic activity. Residue located interior globe is involved in formation of hydrophobic core. Change of one hydrophobic side chain to another one should not affect structure and stability of the protein significantly. Cysteines are located within catalytic center of enzymes and there modification could be critical for enzymatic activity. Amidation of Glu causes loss of charge this might impact on catalytic center, allosteric regulation and stability as well.

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be the first!

Leave a comment

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS