Answer to Question #39635 in Biochemistry for Jazz

Question

1. When preparing the protein standards, we used a tube (T) without protein, but
including Bradford reagent and an equivalent volume of 0.15 M NaCl instead of
protein. Can we just use plain water to prepare tube T instead?

2. The objective of this exercise was to train you in determining protein concentration
in different extracts from biological samples. The protein concentration in a
particular sample is used to standardize the activity of a protein such as an enzyme,
a transporter, or receptor. Define the term “specific activity” in regards to an
enzymatically catalyzed reaction.