Answer to Question #329250 in Biochemistry for aun

Question

Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as

b-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in

Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-

ml solution containing 10-9 g of purified penicillinase was measured as a function of the

concentration of penicillin. Assume that the concentration of penicillin does not change

appreciablyduring the assay.

(a) Plot V0 versus [S] and 1/V0 versus 1/[S] for these data. Does penicillinase appear to obey

Michaelis-Mentenkinetics? If so, what is the value of KM?

(b) What is the valueof V max?

(c) What is the turnover number of penicillinase under these experimental conditions?

Assume one active site per enzyme molecule