Answer to Question #314524 in Biochemistry for Mty

Question

Calculate the Vmax and Km for all reactions in Table 4 and classify type of inhibition for both compounds.

Table 4

No compound (Reference)

[S] - [V]

1 - 0.0510

2 - 0.0720

4 - 0.0950

6 - 0.0150

8 - 0.1110

10 - 0.1070

12 - 0.1120

Compound A

[S] - [V]

1 - 0.0397

2 - 0.0568

4 - 0.0724

6 - 0.0798

8 - 0.0840

10 - 0.0868

12 - 0.0887

Compound B

[S] - [V]

1 - 0.0333

2 - 0.0529

4 - 0.0749

6 - 0.0870

8 - 0.0947

10 - 0.0999

12 - 0.1038

Answer 1

The rate equation for an enzyme subject to competitive inhibition is

V0=Vmax[S]αKm+[S]

V0=Vmax[S]αKm+[S]

,Beginning with a new definition of total enzyme as

[Et]=[E]+[ES]+[EI]

[Et]=[E]+[ES]+[EI]

and the definitions of a

a and K1K1 provided in the text, derive the rate equation above. Use the derivation of the Michaelis-Menten equation as a guide.