Answer to Question #264532 in Biochemistry for casanova

Question

Suggest a pH of a buffer that can be used in an electrophoresis experiment to ensure that the amino acid asparagine will migrate to the cathode portion of the matrix. Explain your answer briefly.

Answer 1

A pH of (8 to 8.5) will be good for buffer in electrophoresis experiment. For eg- TAE or TBE, both of these are buffer containing EDTA, Buffer maintains the pH of medium by which nucleic acid can run smoothly. EDTA is chelating agent bind with divalent cations specially Mg, which is required for action of DNase enzyme. In absence of free Mg++ DNase is inactive, so it can not degrade DNA.