Answer to Question #206776 in Biochemistry for elaine

Question

1. Explain the effects of triton on the activities of each of the enzymes (MDH and LDH).
2. Explain the observed distribution of the enzymes in terms of their metabolic roles.

Answer 1

1.On addition of triton to both enzymes MDH and LDH it renatures them.

2. Enzymes are proteins that act as biological catalysts (biocatalysts). Catalysts accelerate chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps. Enzymes can be involved at each step, where the molecule is transformed into another form, due to the presence of a specific enzyme. Such a reaction pathway can create a new molecule (biosynthesis), or it can break down a molecule (degradation).