Answer to Question #174558 in Biochemistry for Muhammad-Umar Badat

Question

 Q3. The Trypsin-EDTA solution used in the experiment would contain 0.4% trypsin (w/v) and

0.03% EDTA (w/v). It is provided to you as a 10x stock solution.  

(a) EDTA has a molecular weight of 292.24. What is the molarity of the EDTA in the 10X stock solution? (3 marks)

(b) What is the total final amount (in mg) of Trypsin which is added to the cells? (2 marks) 

Answer 1

a) 1.027 mM

b) $m = (\frac{\frac {0.4 g} {100}} {10}) = 0.0004 g = 0.4 mg$