Answer to Question #170826 in Biochemistry for Mayuka

Question #170826

Connect the terminologies DISEASE FORMATION and NUCLEIC ACIDS.

1
Expert's answer
2021-03-11T07:14:05-0500

The use of nucleic acid detection for the diagnosis of genetic and infectious diseases in clinical laboratories was possible after the advent of PCR , a technique based on amplification of nucleic acids by means of thermostable polymerase enzymes and a thermocycler. By this method, typically, DNA duplex templates are melted at high temperatures, and two oligonucleotides complementary to the flanking gene target sequence are specifically annealed in a strict temperature dependent on the primer sequence and length.


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