Question

Connect the terminologies DISEASE FORMATION and NUCLEIC ACIDS.

Answer 1

The use of nucleic acid detection for the diagnosis of genetic and infectious diseases in clinical laboratories was possible after the advent of PCR , a technique based on amplification of nucleic acids by means of thermostable polymerase enzymes and a thermocycler. By this method, typically, DNA duplex templates are melted at high temperatures, and two oligonucleotides complementary to the flanking gene target sequence are specifically annealed in a strict temperature dependent on the primer sequence and length.