Answer to Question #138119 in Biochemistry for Dipti

Question #138119

How will you prepare 250ml of 10X TBE Buffer stock solution using 1M Tris-Cl and

0.5m EDTA?


1
Expert's answer
2020-10-15T06:43:36-0400

You will need to put 100mL of distilled water in a beaker with a magnetic stir bar inside. Add 25mL of the provided 1M Tri-Cl and 1mL of 0.5M EDTA into the water and stir thoroughly.


Pour the mixture into a 250mL volumetric flask rinsing the beaker thoroughly with 20mL of distilled water and adding it into the flask.


Dilute with distilled water to the mark.


Any undissolved white clumps can be redissolved by placing the volumetric flask in a hot water bath.



Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

Dipti
15.10.20, 14:16

Thank you!

Leave a comment

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS