Answer to Question #126385 in Biochemistry for Tom

Question

How to determine the kJ of energy obtained from 1 mole of NADH?

Answer 1

The net gain from one cycle is $3NADH$ and $1FADH_2$ as hydrogen- (proton plus electron)-carrying compounds and $1$ high-energy GTP, which may subsequently be used to produce ATP. Thus, the total yield from $1$ glucose molecule ($2$ pyruvate molecules) is $6NADH$$2FADH_2$ , and $2ATP$

The net equation for the hydrolysis reaction is;

$Glucose+2NAD^+2p_i+2NADP\to 2pyruvate+2H^++2NADH+ 2ATP+2H^++2H_2O+energy$

Calculating the amount of energy released per mole involves the following steps:

The first step involves the determination of moles of a solute.

$q= m*C_g*\Delta T.q$

Whereby q= Amount of energy released /absorbed

m= mass

Cg= Specific heat capacity

$\Delta$T= Change in temperature

To determine the moles of a solute $=n$ $=$ $m\over M$

Where n=moles of absolute

m= moles of absolute

M= Molar mass of absolute

To determine the amount of energy(heat) released or absorbed per mole of solute is calculated as

$\Delta_H$ $_{soln}$ $=$ $q\over n$

Where $\Delta_H$ = Molar enthalpy (heat) of solution

q= Amount of energy(heat)released /absorbed

n= moles of solute