Answer to Question #118060 in Biochemistry for Yaa

Question

Elucidate two physical properties that will enable you to isolate TOL plasmid from the chromosomal DNA of the bacteria

Answer 1

The TOL plasmid encodes enzymes for conversion of the aromatic hydrocarbons to the corresponding carboxylic acids as well as the meta-pathway enzymes. The structure of the promoter sequences involve in degradation of toluene. These are upper- and meta-pathway operon promoters and promoters of the two regulatory genes xylS and xy/R. The TOL- encoded regulators are the enhancer binding protein XylR, which controls the upper pathway Pu and xylS gene P_S1 σ⁵⁴-dependent promoters, and the AraC family regulator XylS, which controls the σ³²−σ³⁸-dependent meta-cleavage pathway promoter Pm. Based on these properties, we can use DNA polymerase specifically labeled with fluorescent dyes. As a result of the attachment of this polymerase near regulatory elements, it is possible to isolate and distinguish the plasmid from the rest of the genetic material in any sort of section.