Microbiology Answers

Why are the abiotic and biotic factors different at high tide and low tide in a mangrove ecosystem?

Would increasing RepA production increase copy number? (2 marks)
1. Yes, because RepA in this system is needed to increase initiation at the ori.
2. No, because RepA in this system is involved in “handcuffing”.

Create an E. coli cell that does not allow for replication of the incA-containing P1 plasmid,
but still replicates its own DNA normally. (5 marks)
1. Add a gene to the chromosome that expresses an iteron-binding protein at high levels.
2. Add a gene to the chromosome that expresses a RepA binding protein that prevents RepA from
binding to the inc sites.
3. Add a gene that encodes a countertranscript to repA mRNA.
4. Add a gene that binds the host DnaG primase, inactivating it

The following is the sequence of a mRNA. 5’ AAGUUCGC . Which would be the template
to encode an RNA sequence would act as its countertranscript? (6 marks)
1. 5’ AAGUUCGC
2. 5’AAGUUCGC
3. 5’ AAGTTCGC
4. 5’GCGAACTT
5. 5’GCGAACUU